A report of three cases of diabetic nephropathy satisfactorily treated with traditional herbal medicine

We reported that three cases of diabetic nephropathy, who had overt proteinuria, were treated satisfactorily not only for their symptoms but also renal nephropathy with traditional herbal medicine over the long term. In Case 1, the level of S-Cr had changed from 1.2 to 2.0 mg/dl over 98 months, in Case 2 from 0.9 to 1.9 mg/dl over 70 months, and in Case 3 from 1.1 to 2.9 mg/dl over 81 months, acceptably moderate increases, meaning that the progression of their renal insufficiency was controlled over the long term. At the same time, the symptoms of hotness, numbness and edema were improved. Diabetic nephropathy with overt proteinuria is known to develop into renal failure after several years. In these cases, traditional herbal medicine was considered responsible for improving their QOL and prolonging the pre-dialysis period of diabetic nephropathy. 顕性蛋白尿期にある糖尿病性腎症の患者に漢方治療を施行した。その結果,随伴する諸症状の改善のみならず長期問,腎機能低下の進行を抑制した3症例を経験したので報告した。症例lでは,98ケ月の観察でS-Cr値はl.2から2.0mg/dl,症例2では70ケ月の観察で,S-Cr値は0.9から1.9mg/dl,症例3では81ケ月の観察で,S-Cr値は1.1から2.9mg/dl,と長期間腎機能低下の進行を抑制した。同時に,手足の火照りやしぴれ感,下肢の浮腫などの症状の軽快も認めた。持続的に蛋白尿を認める顕牲腎症期に至った糖尿病性腎症は数年の経過で,末期腎不全から血液透析に至ると言われている。今回の症例から和漢薬は,糖尿病性腎症における腎機能障害に対して腎機能障害の進行抑制作用を有する可能性が示唆された。このことから,和漢薬は糖尿病に随伴する諸症状を緩和し,QOLを改善するのみでなく,血液透析導人までの期間を延長し,予後を改善したと考えられた

Immunological analysis into Kl-disorder based on traditional Japanese Oriental diagnostic system

漢方医学での病態を把握し有効方剤を選択するためには,伝統医学的手法を応用することが,現在でも最も客観的な方法であると考えられる。しかしながら,伝統医学的手法は心身・如を助炭とし,きわめて全人的な指標を中心としているが故に,西洋医学的な客観性,すなわち要素還元性に乏しいと指摘されている。この研究では気の異常を臨床免疫学的手法で客観化することを目的とした。22名の関節リウマ患者の気虚,気鬱,気逆の病態と末梢血リンパ球のサブセット(CD3, CD19, CD4,CD8, CD16, CD56, CD158a, CD158b)の関連を検討した。その結果,気虚とCD16+CD158b+cellのpopulationが有意な負の相関を示し,気虚を伴う患者群ではCD16+CD158b+cellのpopulationが少ない傾向にあった。この逆相関の関係は4名の3ヶ月の追跡研究でも観察された。このことから活性化したXK細胞の減少は,気虚の病態を呈した患者の一つの特徴と考えられる。このような検討から気虚など複雑な要素が関与する病態の一部を客観化して同一症例での経過観察などに応用可能と考えられる。ただし複雑性の科学の観点から診断への応用に関しては制限される必要がある。 Although traditional medicine is an all-around medical service, it has been pointed out that traditional medical procedures are not objective, and are independent of reductionism. The aim of this study was to determine the immune status of the patients with Kl-disorder. We assessed the association between Kl-disorder and the population of peripheral lymphocytes (CD3, CD19, CD4, CD8, CD16, CD56, CD158a/b:killer cells immunoglobulin-like receptors) in 22 patients with rheumatoid arthritis. The results showed a negative correlation between the score of Kl-deficiency and the population of the CD16+CD158b+cells, and also showed that patients with Kl-deficiency have a tendency to have a reduced population of CD16+CD158b+cells. The inverse relationship between these was also observed in a 3-month follow-up study. Thus, it is possible, in part, to assess the condition of Kl-deficiency objectively using the population of CD16+CD158b+cells. However, the diagnostic utility of CD158b+expressing cells is expected to be limited from the viewpoints of complexity

Effect of Keishi-bukuryo-gan on asymptomatic cerebral infarction for short term

無症候性脳梗塞患者に対する桂枝茯苓丸の効果を検討した。142例(男性32例,女性110例,平均年齢68.9才)を対象とし,副作用のため内服を中止した3例を除く139例に対して,桂枝茯苓丸エキス1日量7.5gを12週間投与した。投与開始時と比較して,改訂長谷川式簡易知能評価スケール,Apathyスケール(やる気スケール),SDS(うつ状態スケール)は有意に改善した。また,頭重感,頭痛,めまいの自覚症状も桂枝茯苓丸の投与により改善し,拡張期血圧は投与前に比べ有意に低下した。これらの結果から,桂枝茯苓丸が無症候性脳梗塞に伴う認知機能の低下,精神症状や自覚症状の悪化に対して有効である可能性が示唆された。 The efficacy of Keishi-bukuryo-gan in patients suffering from asymptomatic cerebral infarction was studied. 142 patients, 32 males and 110 females, with a mean age of 68.9 years, were enrolled and analyzed, and 139 completed the study. They were given Keishi-bukuryo-gan extract (7.5g/day) three times a day for 12 weeks. In comparison to the beginning of the study, Keishi-bukuryo-gan showed improvement with statistical significance in the mean revised version of Hasegawa\u27s dementia scale, Apathy scale and Self-rating depression scale. The number of patients with subjective symptoms as headdullness, headache and dizziness decreased by the treatment with Keishi-bukuryo-gan, and diastolic blood pressure was decreased with statistical significance as compared to the beginning of the study. These results suggest that Keishi-bukuryo-gan is effective against dysfunction of acknowledgment, emotional disorder and subjective symptoms with asymptomatic cerebral infarction

Identifying Potential Problems and Risks in GQM+Strategies Models Using Metamodel and Design Principles

Although GQM+Strategies®1 assures that business goals and strategies are aligned throughout an organization and at each organizational unit based on the rationales to achieve the overall business goals, whether the GQM+Strategies grid is created correctly cannot be determined because the current definition of GQM+Strategies allows multiple perspectives when aligning goals with strategies. Here we define modeling rules for GQM+Strategies with a metamodel specified with a UML class diagram. Additionally, we create design principles that consist of relationship constraints between GQM+Strategies elements, which configure GQM+Strategies grids. We demonstrate that the GQM+Strategies grids can be automatically determined with the help of design principles described in OCL. In fact, an experiment is implemented using these approaches in order to show that this method helps identify and improve potential problems and risks. The results confirm that our approaches help create a consistent GQM+Strategies grid

Repeated X-RAY Irradiation Generates the Radioresistant Cancer Cell that Underwent Epithelial to Mesenchymal Transition

Cancer cell that relapsed after radiotherapy must acquires radioresistance, but the mechanisms of acquired radioresistance in cancer cell are not fully understood. To investigate the mechanisms of acquired radioresistance in cancer cell, we irradiated the human adenocarcinoma cell line A549 with 2Gy of X-ray, repeated the irradiation 30 times, and established the radioresistant cancer cell line A549R. To evaluate the differences in the radioseisitivity, apoptosis and senescence induction between A549 and A549R, we performed colony formation assay, JC-1 staining, and X-gal staining, respectively. The results of colony formation assay showed that the survival fraction of A549R was significantly higher than that of A549. In addition, apoptosis and senescence induction in A549R were suppressed compared with that in A549. We further studied the DNA double strand break (DSB) repair potential in A549R. To measure the DSB repair potential, we performed the immunofluorescence staining with anti-gamma-H2AX, phosphorylated ataxia telangiectasia mutated (p-ATM) antibody at 1 to 3 days after X-ray irradiation and counted the focus number of gamma-H2AX and p-ATM in the nucleus. As a result, the disappearance of colocalized focus of gamma-H2AX and p-ATM in A549R was earlier than that in A549. These data showed that the repeated X-ray irradiation generates the radioresistant cancer cells that acquire the promotion of DSB repair potential. Next, we evaluated the difference in cell cycle progression and cell proliferation after X-ray irradiation between both cell lines. As a result, the percentage of G0/G1 phase in A549R was significantly higher than that in A549. Moreover, the cell proliferation in A549R after X-ray irradiation was suppressed compared with that in A549. Subsequently, we assessed the protein expression of p53 and p53-related proteins such as Bax, Puma, p21, and cyclin D1 by western blotting. The results showed that the p53 and cyclin D1 expression in A549R was suppressed compared with that in A549, but Bax, Puma, and p21 was not changed.We further assessed whether epithelial to mesenchymal transition (EMT) in A549R because it is known that EMT is closely associated with chemoresistance in cancer cell. As we expected, the cadherin switching was clearly observed in A549R. In this report, we will discuss the relationship between the radioresistance after X-ray irradiation, changes in DNA repair potential, and EMT induction.8th International Symposium of Cancer Research and Therap